Sipunculus nudus SSR primer group and application thereof

• Main Authors: YANG JIALIN, PENG HUIJING, YANG TINGTING, HUANG XIUYI, WANG PENGLIANG, ZOU JIE, HAN CHUNLI, YANG GUOHAO, BAO LEI
• Format: Patent
• Language: Chinese; English
• Published: 10.05.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of molecular markers, in particular to a sipunculus nudus SSR primer group and application thereof.The sipunculus nudus serves as a material, DNA of the sipunculus nudus is extracted and detected and analyzed to obtain microsatellite fragments, microsatellite markers with high polymorphism are developed and screened out, the genetic diversity of sipunculus nudus populations in the northern bay coastal area can be revealed, and the application of the sipunculus nudus SSR primer group is developed. Corresponding SSR primers are obtained, an SSR primer group suitable for being applied to sipunculus nudus clustering analysis is found, the primer group has a high PIC value, PCR analysis is conducted by means of the SSR primer group, a clustering analysis chart is drawn, and the accuracy of clustering analysis is improved. And new primers and thoughts are provided for research on genetic breeding, gene positioning, species genetic relationship identification, sipunculus