Centrifugal digital PCR (Polymerase Chain Reaction) chip for high-throughput typing detection and use method

• Main Authors: GAO CHANG, CHU JINYU, XU TING, ZHUANG TIANCHI, JI MINGHUI, YANG YINGQI, YU YUE, MEI HAOKUN
• Format: Patent
• Language: Chinese; English
• Published: 10.05.2024
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TRANSPORTING; VINEGAR; WINE

The invention discloses a centrifugal digital PCR (polymerase chain reaction) chip for high-throughput typing detection of samples, which comprises a circular chip body, a center shaft mounting hole is formed in the chip body, the chip body is divided into more than two fan-shaped areas, a liquid buffer tank, a liquid storage tank and a droplet generation unit are sequentially arranged on each fan-shaped area from the center to the outer edge, and the droplet generation unit is connected with the center shaft mounting hole. A sample adding hole is formed in an inlet of the liquid buffering pool, the liquid storage pool is of an arc-shaped groove structure, at least two liquid outlets facing the outer edge of the chip body are formed in the liquid storage pool and distributed on the arc-shaped groove at intervals, and each liquid outlet is connected with a microdroplet generation unit. Each micro-droplet generation unit comprises a first micro-fluidic channel, a primer chamber, a second micro-fluidic channel,