Carrier for promoting transfection of in-vivo cationic lipid-nucleic acid complex through ultrasonic cavitation as well as preparation method and application of carrier

• Main Authors: WANG GUOWEI, QIU CHEN, JIN PEILE, HUANG PINTONG, CHEN JIFAN, LIN TAO
• Format: Patent
• Language: Chinese; English
• Published: 10.05.2024
• Subjects: HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS

The invention discloses a carrier for promoting in-vivo cationic lipid-nucleic acid complex transfection through ultrasonic cavitation and a preparation method and application thereof.The carrier comprises microbubbles, a cationic lipid-nucleic acid complex and protein, and the cationic lipid-nucleic acid complex is adsorbed to the surfaces of the microbubbles; the protein and the cationic lipid-nucleic acid complex are in a combined state through electrostatic adsorption, and under the action of ultrasonic cavitation, the protein and the cationic lipid-nucleic acid complex are transiently separated, so that the transfection performance of the cationic lipid-nucleic acid complex is activated. The carrier provided by the invention is high in stability and low in toxicity after intravenous injection, the cationic lipid-nucleic acid complex and protein can be temporarily removed through ultrasonic cavitation, the difficulty of in-vivo application of the cationic lipid-nucleic acid carrier is overcome, and direct