Primer probe combination for detecting copy number of human motor neuron survival gene SMN1 and application

• Main Authors: QIN WEI, XIANG HONGYUN, QIU GENG, XU LIAN, PENG DANFANG, YE JIANMING
• Format: Patent
• Language: Chinese; English
• Published: 07.05.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a primer probe combination for detecting the copy number of a human motor neuron survival gene SMN1 and application of the primer probe combination. The primer probe combination for detecting the copy number of the human motor neuron survival gene SMN1 comprises a primer pair and a probe for specifically amplifying and detecting the human motor neuron survival gene SMN1, and the primer pair for specifically amplifying the human motor neuron survival gene SMN1 comprises a forward primer and a reverse primer, the method comprises the step of judging the copy number of a target gene by using a digital PCR method. The primer amplification efficiency is high, the probe specificity is strong, the method is simple and convenient, the result is accurate and reliable, and the copy number of the SMN1 gene can be effectively detected. 本发明提供一种用于人运动神经元存活基因SMN1拷贝数检测的引物探针组合及其应用。所述用于人运动神经元存活基因SMN1拷贝数检测的引物探针组合包括特异性扩增并检测人运动神经元存活基因SMN1的引物对和探针，特异性扩增所述人运动神经元存活基因SMN1的引物对包括正向引物和反向引物，所述方法包括应用数字PCR方法判断靶基因拷贝数。本发