Method for producing human chimeric antigen receptor T cells enriched in stem cell-like central memory phenotype T cells

The invention provides a method for producing human chimeric antigen receptor T cells rich in stem cell-like central memory phenotype T cells (Tscm). The method comprises the following steps: providing human peripheral blood mononuclear cells; purifying human T cells: adding magnetic beads containin...

Full description

Saved in:
Bibliographic Details
Main Authors LIN XUANHUI, DONG ZIXUN, LIN JIANTING, LAI YIHUI, LIN CHENGLONG
Format Patent
LanguageChinese
English
Published 07.05.2024
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Online AccessGet full text

Cover

More Information
Summary:The invention provides a method for producing human chimeric antigen receptor T cells rich in stem cell-like central memory phenotype T cells (Tscm). The method comprises the following steps: providing human peripheral blood mononuclear cells; purifying human T cells: adding magnetic beads containing anti-CD3/CD28 antibodies on the surfaces into human peripheral blood mononuclear cells to obtain a human T cell and magnetic bead compound; activating the human T cells: culturing and activating the human T cells and the magnetic bead compound to obtain an activated human T cell and magnetic bead compound; lentivirus transfection: adding lentivirus into the activated human T cell and magnetic bead compound, and transfecting to obtain a human CAR-T cell and magnetic bead compound; removing the magnetic beads: removing the magnetic beads from the compound of the human CAR-T cells and the magnetic beads to obtain the human CAR-T cells; and human CAR-T cell amplification: carrying out amplification culture on the hum
Bibliography:Application Number: CN202211368899