Application of knockout mhpA gene in improvement of yield of L-phenylalanine

• Main Authors: XIE RUFEI, XIE GUOAN, LIANG HENGYU, HAN CHAO, TAN WEI, ZHANG HAO, CHEN MINLIANG, LIU YONGYU
• Format: Patent
• Language: Chinese; English
• Published: 07.05.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses application of a knockout mhpA gene in increasing the yield of L-phenylalanine, and belongs to the technical field of gene engineering. According to the application of the knockout mhpA gene in improving the yield of the L-phenylalanine, disclosed by the invention, the mhpA gene of escherichia coli PHE01 with high yield of the L-phenylalanine is accurately knocked out by utilizing a CRISPR/Cas9 technology, and the yield of the L-phenylalanine of a PHE01 strain is remarkably improved. 本发明公开了敲除mhpA基因在提高L-苯丙氨酸产量中的应用，属于基因工程技术领域。本发明公开的敲除mhpA基因在提高L-苯丙氨酸产量中的应用，利用CRISPR/Cas9技术精准敲除了L-苯丙氨酸高产大肠杆菌PHE01的mhpA基因，并使PHE01菌株的L-苯丙氨酸产量得到显著提升。