Application of F1/F3 polypeptide in preparation of drugs for inducing pyroptosis of tumor cells

• Main Authors: LUO YUANDONG, NI GUOYING, LIU XIAOSONG, WANG TIANFANG
• Format: Patent
• Language: Chinese; English
• Published: 07.05.2024
• Subjects: HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS

The invention belongs to the technical field of biology, and particularly relates to application of F1/F3 polypeptide in preparation of drugs for inducing pyroptosis of tumor cells. It is proved for the first time that natural polypeptide F1, F3 polypeptide or modified F1 polypeptide can induce pyroptosis of tumor cells by activating a Caspase 3/GSDME pathway: when the tumor cells die, a cell membrane bulges to form a bubble, then the bubble is broken, and a large amount of interleukin 18 and LDH are secreted. And when F1 and F3 are combined for use, the effect is optimal. Experiments prove that the secretion amount of the IL18 is in a time and dose dependent relationship. The tumor cells are epithelial tumor cells and comprise cervical cancer cell line Hela cells, lung cancer A549 cells, liver cancer Huh-7 cells and the like. The invention deeply explores the mechanism of the natural polypeptide for preparing the medicine for treating tumors, and lays a solid foundation for further expanding the application