B-cell expansion method for cell therapy

• Main Authors: SELBY MARK J, KOTHAKOTA, SUDHAKAR, BRENNAN THOMAS
• Format: Patent
• Language: Chinese; English
• Published: 03.05.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention disclosed herein relates to improved methods for expanding cell populations, in particular B cell populations. The invention further relates to improved B cell expansion media, compositions comprising expanded B cells and methods of using such expanded B cells. The invention further relates to methods of treating a disease or condition in which a population of B cells is obtained and cultured, and in which the B cells are engineered to express a payload and/or chimeric receptor, and in which the B cells are administered to a subject. 本文所公开的本发明涉及用于扩增细胞群体，具体地B细胞群体的经改进的方法。本发明进一步涉及经改进的B细胞扩增培养基、包括经扩增的B细胞的组合物以及使用此类经扩增的B细胞的方法。本发明进一步涉及治疗疾病或病症的方法，其中获得并且培养B细胞群体，并且其中所述B细胞被工程化以表达有效载荷和/或嵌合受体，并且其中所述B细胞被施用于受试者。