Method for positioning agmatine in plant body and identifying binding protein

• Main Authors: LI YONGBO, CHENG DUNGONG, CUI DEZHOU, FAN QINGQI
• Format: Patent
• Language: Chinese; English
• Published: 03.05.2024
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to the technical field of biology, in particular to a method for positioning agmatine in a plant body and identifying binding protein. A fluorescein isothiocyanate (FITC)-agmatine chemical cross-linked substance is applied to the roots of wheat, and then in-vivo positioning and binding protein identification can be rapidly and accurately performed on agmatine by utilizing an FITC antibody immunoprecipitation-mass spectrometric detection method; therefore, a methodological basis is provided for researching a drought-resisting and yield-increasing mechanism of agmatine. 本发明涉及生物技术领域，具体涉及一种胍丁胺在植物体内定位及结合蛋白鉴定的方法。通过小麦根部施加异硫氰酸荧光素(FITC)-胍丁胺化学交联物，然后利用FITC抗体免疫沉淀-质谱检测的方法可快速、准确对胍丁胺进行体内定位及结合蛋白的鉴定；进而为研究胍丁胺的抗旱增产机理提供方法学基础。