Method for preparing sweet potato virus-free seedlings through axillary bud differentiation

The invention belongs to the technical field of agriculture, and particularly relates to a method for efficiently preparing sweet potato virus-free seedlings through axillary bud differentiation. According to the method, a sweet potato representative variety 'watermelon red' is adopted as...

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Bibliographic Details
Main Authors WEI ZHOUYING, QIAO YU, YE YUWEN, YANG ZHUO, RAN JIAJUN, DAI LEI, CHEN CHAOWU
Format Patent
LanguageChinese
English
Published 03.05.2024
Subjects
AGRICULTURE
ANIMAL HUSBANDRY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
NEW PLANTS OR PROCESSES FOR OBTAINING THEM
PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
TRAPPING
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Summary:The invention belongs to the technical field of agriculture, and particularly relates to a method for efficiently preparing sweet potato virus-free seedlings through axillary bud differentiation. According to the method, a sweet potato representative variety 'watermelon red' is adopted as a material, robust stem segments of 'watermelon red' cultivated in the open air are selected, leaves are removed, and sterile stem segments are obtained after disinfection. The stem segments are inoculated to an axillary bud differentiation formula to be cultured for 20 days, and at least two or more axillary buds can be obtained from each stem node. Stripping shoot tips of the sterile seedlings of watermelon red, and inoculating the shoot tips to a stem tip meristem formula to obtain regenerated seedlings. And carrying out virus detection on the regenerated sweet potatoes to obtain virus-free sweet potato seedlings. The virus-free seedlings are inoculated on a clustered plant formula for growth culture, and large-batch swee
Bibliography:Application Number: CN202410124500