Modified eggshell membrane, immobilized enzyme and application thereof

• Main Authors: HUANG ZHIQIANG, LI CHOU, CHEN JIAMIN, JI XURUI, FENG LIN, GENG RONGQING, JI QINGZHI
• Format: Patent
• Language: Chinese; English
• Published: 30.04.2024
• Subjects: AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F,C08G; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; COMPOSITIONS OF MACROMOLECULAR COMPOUNDS; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; GENERAL PROCESSES OF COMPOUNDING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC MACROMOLECULAR COMPOUNDS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR PREPARATION OR CHEMICAL WORKING-UP; VINEGAR; WINE; WORKING-UP

The invention belongs to the technical field of enzyme immobilization, and particularly relates to a modified eggshell membrane, an immobilized enzyme and application thereof. The modified eggshell membrane is prepared according to the following steps: mixing a sulfydryl-containing substance with an eggshell membrane, and carrying out vulcanization reaction to obtain the eggshell membrane with the sulfydryl-containing surface; mixing the eggshell membrane containing sulfydryl on the surface with glutaraldehyde for reaction to obtain a modified eggshell membrane containing aldehyde on the surface. The modified eggshell membrane can be used for immobilizing an enzyme, an aldehyde group on the eggshell membrane and an amino group of the enzyme are subjected to a Schiff reaction, the enzyme is immobilized, and the enzyme is high in immobilization rate, firm in combination and not prone to falling off. 本发明属于酶固定技术领域，具体涉及一种改性蛋壳膜、固定化酶及其应用。改性蛋壳膜是按照以下步骤制备得到的：将含巯基的物质与蛋壳膜混合进行硫化反应，得到表面含巯基的蛋壳膜；将表面含巯基的蛋壳膜与戊二醛混合进行反应，得到表面含醛基的