Method for inducing transgenic hairy roots of stylosanthes guianensis and method for preparing protoplast

• Main Authors: SUN HAO, ZHAO XINGKUN, LIU PANDAO, ZHANG JIANYU
• Format: Patent
• Language: Chinese; English
• Published: 26.04.2024
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses a method for inducing transgenic hairy roots by stylosanthes guianensis and a method for preparing protoplasts. The method for inducing the transgenic hairy roots by stylosanthes guianensis comprises the following steps: obtaining explants: cutting cotyledonary nodes of germinated stylosanthes guianensis seedlings as the explants; infection and co-culture: soaking the explant in a recombinant agrobacterium rhizogenes Ar1193 infection solution carrying a target gene to obtain an infected explant, sucking the infection solution on the surface of the explant, and placing the explant on an MS solid culture medium for dark culture; inducing and screening: after the dark culture is completed, absorbing liquid residues on the surface, and inserting the explants into an induction culture medium added with antibiotics for inducing and screening to preliminarily obtain transgenic hairy roots; and transferring the transgenic hairy roots into a subculture medium for subculture. According to the me