Brassinolide signal pathway transcription factor for regulating formation of poplar wood and application of brassinolide signal pathway transcription factor

• Main Authors: ZHOU CHONG, DU JUAN, YE TIANQI, SHOU HUIXIA, XU MINGLING
• Format: Patent
• Language: Chinese; English
• Published: 26.04.2024
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; ORGANIC CHEMISTRY; PEPTIDES; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention relates to a brassinolide transcription factor for regulating formation of poplar wood and application of the brassinolide transcription factor, and belongs to the technical field of plant genetic engineering. The nucleotide sequence is shown as a sequence 1 in a sequence table, and the amino acid sequence is shown as a sequence 2 in the sequence table. The PagBRRF1 gene is transferred into the poplar, and compared with a wild type, the transgenic poplar with overexpression of the PagBRRF1 has the advantages that the plant height is obviously increased, the diameter is also increased, and the number of xylem cells is obviously increased. Therefore, the PagBRRF1 gene is a key regulatory gene for regulating the generation of poplar wood, and has important application value in the fields of forest gene engineering and clonal forestry. 本发明涉及一种调控杨树木材形成的油菜素内酯转录因子及其应用，属于植物基因工程技术领域；其核苷酸序列如序列表中的序列1所示，其氨基酸序列如序列表中序列2所示。本发明将PagBRRF1基因转入杨树，过量表达PagBRRF1的转基因杨树与野生型比较，植株高度明显增加，直径也增加，且木质部细胞数量明显增多。说明PagBRRF1基因是调控杨