Liquid chromatographic analysis method for oligonucleotide diastereoisomers

• Main Authors: LIU TING, CAI SONGYAN, HOU LIQIANG, LI ZEYA
• Format: Patent
• Language: Chinese; English
• Published: 19.04.2024
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides an oligonucleotide diastereoisomer liquid chromatography analysis method, which adopts an ion exchange (IEX) chromatographic column, a mobile phase A is 80mM Tris/Cl, the pH value is 7.5, and the concentration is 10% ACN; the mobile phase B is 80 mM of Tris/Cl, 1 M of NaCl and...