Liquid chromatographic analysis method for oligonucleotide diastereoisomers

• Main Authors: LIU TING, CAI SONGYAN, HOU LIQIANG, LI ZEYA
• Format: Patent
• Language: Chinese; English
• Published: 19.04.2024
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides an oligonucleotide diastereoisomer liquid chromatography analysis method, which adopts an ion exchange (IEX) chromatographic column, a mobile phase A is 80mM Tris/Cl, the pH value is 7.5, and the concentration is 10% ACN; the mobile phase B is 80 mM of Tris/Cl, 1 M of NaCl and 10% of ACN; the elution gradient is 0 min, 95% of a mobile phase A: 5% of a mobile phase B; 30 min, 10% of a mobile phase A: 90% of a mobile phase B; 40 min, 10% of a mobile phase A and 90% of a mobile phase B; the mobile phase A is 95%, the mobile phase B is 5%, and the mobile phase A is 95%, the mobile phase B is 5%, and the mobile phase B is 50 min. The method adopts IEX high performance liquid chromatography for analysis, has the advantages of good component peak separation effect, high analysis precision, simplicity and convenience in operation and the like, and achieves the purpose of effectively controlling the quality of oligonucleotide. 本发明提供一种寡核苷酸非对映异构体液相色谱分析方法，采用离子交换(IEX)色谱柱，流动相A为80mM Tris/Cl，pH 7.5，10％