Primer for detecting pathogenic bacteria of black mouth disease of strongylocentrotus intermedius based on LAMP (loop-mediated isothermal amplification)

• Main Authors: ZHAN YAOYAO, ZHAO TANJUN, CHANG YAQING, MAI WENHONG
• Format: Patent
• Language: Chinese; English
• Published: 19.04.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a primer for detecting pathogenic bacteria of black mouth disease of strongylocentrotus intermedius based on LAMP (loop-mediated isothermal amplification), the DNA (deoxyribonucleic acid) sequences of the primer are shown as SEQ ID NO.1-SEQ ID NO.4. The primer has the advantages of high sensitivity, strong specificity and the like, and can be used for detecting pathogenic bacteria of black mouth disease of strongylocentrotus intermedius by only needing a constant-temperature water bath kettle. The visual LAMP detection of main pathogenic bacteria (vibrio urchin urchin black mouth disease, vibrio apex, slow vibrio and vibrio splendidus) of the strongylocentrotus intermedius black mouth disease by taking HNB as an indicator can be completed within 1 h under the condition of 68 DEG C, the method is suitable for on-site rapid detection, precise and expensive equipment is not needed, and the detection cost of the pathogenic bacteria of the strongylocentrotus intermedius black mouth disease