Primer probe group for detecting biological distribution of biological product containing human OTOF gene based on qPCR (quantitative polymerase chain reaction) as well as kit and application of primer probe group

• Main Authors: XIA YAN, ZHANG YANLIN, HOU DEBAO, ZHANG BAIQING
• Format: Patent
• Language: Chinese; English
• Published: 19.04.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a primer probe group for detecting biological distribution of a biological product containing a human OTOF gene based on a qPCR technology and a kit and application of the primer probe group, and belongs to the technical field of molecular biological detection. The invention discloses a primer probe group for detecting biological distribution of a biological product containing a human OTOF gene based on a qPCR technology. The primer probe group comprises a forward primer with a nucleotide sequence as shown in SEQ ID NO: 1, a reverse primer with a nucleotide sequence as shown in SEQ ID NO: 2 and an MGB probe with a nucleotide sequence as shown in SEQ ID NO: 3. The invention also provides application of the primer probe group in preparation of a kit for detecting biological distribution of a biological product containing the human OTOF gene. The kit can rapidly and accurately detect the biological distribution of a biological product containing the human OTOF gene, and provides a basis fo