Method for simultaneous in-situ fluorescence detection of human RNA (Ribonucleic Acid), DNA (Deoxyribonucleic Acid) and protein

• Main Authors: YU FUHAI, QIN MENG, QI XINTONG, LU YONGJIE, YUAN PENG, YAN LIYING, HUA LINGYUE, QIAO JIE
• Format: Patent
• Language: Chinese; English
• Published: 16.04.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

The invention belongs to the field of biological detection, and particularly relates to a method for simultaneous in-situ fluorescence detection of RNA (Ribonucleic Acid), DNA (Deoxyribose Nucleic Acid) and protein. A TSA signal amplification system (TSA dye) is adopted to enable RNA-FISH and protein immunofluorescence signals to be retained in high-temperature and strong-acid (pepsase digestion step) environments in a DNA-FISH experimental process, so that original RNA and protein signals are retained, and simultaneous in-situ fluorescence detection of human RNA, DNA and protein is realized. Wide application prospects are realized. 本发明属于生物检测领域，具体的，一种同时原位荧光检测RNA、DNA、蛋白质的方法。本发明采用TSA信号放大系统（TSA染料）让RNA-FISH和蛋白免疫荧光的信号可以在DNA-FISH实验过程的高温和强酸（胃蛋白酶消化步骤）环境中保留，从而保留原来的RNA和蛋白信号，实现同时原位荧光检测人类RNA、DNA、蛋白质。具有广阔的应用前景。