Primer combination for detecting respiratory pathogens, kit and application

• Main Authors: CHEN ZHAO, WAN CHONG, LIU XIN, HOU GUANGYUAN, ZHANG YAN, CHEN HUI, XU JUNPU, WANG XUTONG, MO MINLI, ZHANG FENG, LIU YUZHONG
• Format: Patent
• Language: Chinese; English
• Published: 12.04.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMBINATORIAL CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of nucleic acid sequencing, in particular to a primer combination for detecting respiratory pathogens, a kit and application. In the primer combination provided by the invention, each sequence only contains a target nucleic acid sequence of a pathogen or a human genome and does not contain a non-target sequence, so that efficient amplification of pathogen nucleic acid is ensured. Meanwhile, the primer combination provided by the invention has high specificity and high sensitivity in multiple PCR (Polymerase Chain Reaction), and has strong inclusiveness on detection of different pathogen types, and extremely few dimers are formed among multiple pairs of primers, so that the detection of multiple pathogens is more accurate. 本发明涉及核酸测序技术领域，尤其涉及用于检测呼吸道病原体的引物组合、试剂盒及应用。本发明提供的引物组合中，各序列仅含病原体或人基因组的靶核酸序列，不含有非靶序列，确保了病原体核酸的高效扩增。同时，本发明提供的引物组合在多重PCR中具有高特异性和高灵敏度，对不同病原体型别检测的包容性强，多对引物之间形成的二聚体极少，使得对多种病原体的检测更加准确。