Biosensor based on strand displacement reaction and application thereof

• Main Authors: LI PEIRU, GAO EBIN
• Format: Patent
• Language: Chinese; English
• Published: 12.04.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention discloses a biosensor based on a strand displacement reaction. The biosensor comprises a catalytic hairpin CH1, a catalytic hairpin CH2, a Cas12a protein, crRNA and an ssDNA reporter molecule, according to the invention, a novel TMSDR-Cas12a biosensor with high amplification efficiency and cascade amplification of detection signals is constructed on the basis of a constant-temperature and enzyme-free nucleic acid amplification technology of a Toehold-mediated DNA chain replacement reaction and in combination with a CRISPR/Cas12a system, and the technology is high in sensitivity and good in specificity, and can be used for rapid visual detection of trace nucleic acid in a sample at normal temperature. 本发明公开了一种基于链置换反应的生物传感器，所述生物传感器包括催化型发夹CH1、催化型发夹CH2、Cas12a蛋白、crRNA和ssDNA报告分子；本发明基于Toehold介导的DNA链替换反应的恒温、无酶核酸扩增技术，并结合CRISPR/Cas12a系统构建一种新型、高扩增效率且检测信号联级放大的TMSDR-Cas12a生物传感器，该技术灵敏度高、特异性好，常温条件下就能够用于样品中痕量核酸的快速可视化检测。