Enzyme composition, CRISPR-Cas compound preparation and application

• Main Authors: GAO XIAOSHA, NAN YUNQIAN, LI MING, CHEN ZHONGJIAN, FAN SHUO, SUN YANG, PAN WEIYE, PENG QIONGFANG
• Format: Patent
• Language: Chinese; English
• Published: 12.04.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to an enzyme composition, a CRISPR-Cas compound preparation and application, and relates to the field of biological detection. The enzyme composition comprises an enzyme and a protective agent, a reaction substrate of the enzyme is nucleic acid, and the protective agent comprises polyvinyl alcohol and/or soluble starch. The enzyme composition contains a specific protective agent, so that the enzyme can be preserved under the condition of no glycerol protection, recognition of guide RNA can be recovered in the subsequent detection process, the forming efficiency of the CRISPR-Cas compound preparation is improved, the CRISPR-Cas compound preparation with higher activity is formed, the dependence of an enzyme preservation system on glycerol is reduced, and the activity of the CRISPR-Cas compound preparation is improved. And the preservation of the system and the rapid dispersibility during use are facilitated. 本发明涉及酶组合物、CRISPR-Cas复合物制剂及应用，涉及生物检测领域。该酶组合物包括酶和保护剂，酶的反应底物为核酸，保护剂包括聚乙烯醇和/或可溶性淀粉。该酶