Efficient and universal mouse myocardial cell separation buffer solution and application thereof

• Main Authors: QIAO YAN, MENG QINGHANG, MI QIONGJIE
• Format: Patent
• Language: Chinese; English
• Published: 12.04.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the technical field of myocardial cell separation, and discloses an efficient and universal mouse myocardial cell separation buffer solution and a separation method thereof, and the method is simple to operate, short in time consumption, high in myocardial cell separation efficiency, small in influence on cell activity and remarkably improved in success rate. And the Collagenase buffer solution used in the method improves the separation efficiency of the myocardial cells from multiple aspects, can be suitable for separating the myocardial cells of the mice at the age group in a wider range by adjusting different concentration ratios in the components, and realizes the purposes of one object with multiple purposes and cost saving in actual operation. Various experimental requirements of mouse myocardial cells can be met, and the method has wide practical application value. In addition, the method can provide better technical support for studying physiological functions of myocardial ce