Preparation method of metarhizium anisopliae fungicide for biocontrol

The invention belongs to the technical field of preparation of biocontrol microbial agents, and particularly relates to a preparation method of a metarhizium anisopliae microbial agent for biocontrol. The method comprises the following steps: (1) weighing raw materials of a culture medium, wherein a...

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Bibliographic Details
Main Authors SONG WENXIA, HAN XIAOLONG
Format Patent
LanguageChinese
English
Published 12.04.2024
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention belongs to the technical field of preparation of biocontrol microbial agents, and particularly relates to a preparation method of a metarhizium anisopliae microbial agent for biocontrol. The method comprises the following steps: (1) weighing raw materials of a culture medium, wherein a metarhizium anisopliae fermentation culture medium comprises the following raw materials in percentage by mass: 44-48% of rice bran, 18-22% of rice husk, 10-12% of monosodium glutamate mother liquor and 20-24% of water; (2) sterilizing the culture medium: mixing rice bran, monosodium glutamate mother liquor and water, sterilizing, and independently sterilizing rice husk for later use; (3) inoculating: firstly mixing the metarhizium anisopliae strain with the rice husks, and then mixing with other raw materials of the culture medium; (4) briquetting: preparing the inoculated culture medium into culture medium briquettes; and (5) culturing: briquetting the culture medium, culturing, and collecting spores after the c
Bibliography:Application Number: CN202311800699