Breeding method of brassica napus radish cytoplasm restoring line independent of restoring source

• Main Authors: TAO LANRONG, GONG WANZHUO, SHI HAORAN, HE LICHUAN, WU QIAOBO, ZOU QIONG, WANG JISHENG, LI YUN, WU XUEMING, FU SHAOHONG, YANG JIN, YU QIN, LIANG XINGYU
• Format: Patent
• Language: Chinese; English
• Published: 09.04.2024
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING

The invention discloses a breeding method of a brassica napus radish cytoplasm restoring line independent of a restoring source, and relates to the technical field of crop breeding, and the breeding method comprises the following steps: carrying out test cross or multi-generation backcross on brassica napus and a brassica napus radish cytoplasm sterile line to form a radish cytoplasm hybridization F1 generation; or a stable new radish cytoplasmic sterile line; the method comprises the following steps of: pollinating and inducing a rape double haploid induction line which does not contain a radish cytoplasm restoring gene, bagging and screening fertile single plants in offspring obtained by pollination, continuously selfing the screened fertile single plants which are induced to generate the restoring gene for 2-3 generations, then carrying out test cross, and if the test cross offspring is full fertile, carrying out test cross on the fertile single plants. And breeding the stable brassica napus radish cytopla