Methods for sperm sorting using liposomes and/or Slo3 potassium channel inhibitors

• Main Authors: SCHMIDT, EUGEN, CARION OLIVIER
• Format: Patent
• Language: Chinese; English
• Published: 05.04.2024
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS; VINEGAR; WINE

The present invention relates to a method for sperm sorting of sperm cells contained in sperm of a mammalian species, said method comprising the step of mixing a sperm sample with a liposome and/or at least one Slo3 potassium channel inhibitor. Specifically, the invention also relates to a method for sorting sperm cells contained in sperm of a mammalian species, the method comprising the following steps: mixing the sperm and a fluorescent dye for labeling sperm DNA with a sample liquid to form a liquid mixture; providing a flow cytometry sperm sorting device selected from the group consisting of an in-air droplet system and a flow cell tip closure system; -using a sperm sorting device for said labeled liquid mixture, using a high-energy luminescent light source associated with a specific excitation spectrum of the dye, thereby providing each sperm cell entrapped in a single droplet, which droplet is hydrodynamically oriented by using a sheath fluid, identifying the DNA content in said droplet, distributing el