Method for detecting cross reaction of food allergens

• Main Authors: HE XINRONG, LIU HONG, LIU GUANGMING, YU CHENCHEN, JI NAIRU, HAN XINYU
• Format: Patent
• Language: Chinese; English
• Published: 05.04.2024
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides a method for detecting cross reaction of food allergens, which comprises the following steps: establishing a Balb/c mouse model with allergen specificity and food allergy, and analyzing the proliferation condition of specific CD4 + T lymphocytes and the release level of cytokine IL-2 in mononuclear cell culture supernatant; compared with PBS, the to-be-detected food allergen significantly stimulates the proliferation of modeled allergen specific CD4 + T lymphocytes, and the release level of IL-2 is significantly increased, indicating that a cross reaction exists between the to-be-detected food allergen and the specific allergen of the sensitized mouse. Therefore, the method for detecting the cross reaction of the food allergens is successfully established and can be used for detecting the cross reaction of the food allergens. 本发明提供了一种检测食物致敏原交叉反应的方法，该方法包括建立致敏原特异性食物过敏的Balb/c小鼠模型，分析特异性CD4+T淋巴细胞增殖情况和单个核细胞培养上清中细胞因子IL-2的释放水平；与PBS相比，待测食物致敏原显著刺激建模的致敏原特异性CD4+T淋巴细胞增殖，并且IL-2的释放水平显著上升，表明待测食物致敏原与该致敏