Method for immobilizing fructosyl transferase by taking chitosan modified diatomite as carrier

The invention discloses a method for immobilizing fructosyl transferase by taking chitosan modified diatomite as a carrier, and belongs to the technical field of biology. The method for immobilizing fructosyl transferase comprises the following steps: weighing diatomite, and activating the diatomite...

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Main Authors ZHAO ZISHEN, CHEN JINGJING, XIAO ZIQUN, JIANG BO
Format Patent
LanguageChinese
English
Published 05.04.2024
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses a method for immobilizing fructosyl transferase by taking chitosan modified diatomite as a carrier, and belongs to the technical field of biology. The method for immobilizing fructosyl transferase comprises the following steps: weighing diatomite, and activating the diatomite in a dilute sulfuric acid solution for 2-10 hours to obtain diatomite activated by dilute sulfuric acid; then adding diatomite activated by dilute sulphuric acid into a chitosan solution, reacting in a shaking table for 4-10 hours, washing and drying to obtain chitosan modified diatomite; and then dispersing the chitosan modified diatomite in a citric acid-phosphate buffer solution, adding fructosyl transferase, carrying out shaking reaction, and after the reaction is finished, washing and drying to obtain the chitosan modified diatomite. The specific enzyme activity of the immobilized fructosyl transferase obtained by the method can reach 431 U.g <-1 >, the enzyme activity recovery rate and the protein immobiliza
Bibliography:Application Number: CN202311843116