Recombinant cell for screening RXR alpha effector, construction method and application

• Main Authors: SUN ZHENDONG, LIU QIAN, ZHOU QUNFANG, JIANG GUIBIN, YANG XIAOXI, QIN CHAOJIE
• Format: Patent
• Language: Chinese; English
• Published: 05.04.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of biology, particularly discloses a recombinant cell for screening an RXR alpha effector and a preparation method of the recombinant cell, and further provides a method or application of the recombinant cell in screening the RXR alpha effector. An in-vitro monkey-derived kidney cell COS-7 is used as a host cell, a lentivirus transfection Luc reporter gene vector and a human RXR alpha expression vector are used to construct a stably-transfected recombinant cell, and the recombinant cell can be used for evaluating the activation or inhibition effect of an exogenous compound on RXR alpha, and can be used for preparing a human RXR alpha expression vector. Therefore, the method has a good application prospect in the aspects of RXR alpha gene function research, target verification, drug screening, endocrine disrupter (EDCs) screening and the like. The method for carrying out RXR alpha effector in-vitro screening by using the recombinant cell has the advantages of simpli