Method and system for constructing and identifying rhesus monkey intestinal tract organ model

• Main Authors: LIANG GUILI, WU ZAOSHOU, LAN DANFENG, ZHANG ANXING, WANG JUNYU, WU BIAN, MAO FUGANG, LIU YU
• Format: Patent
• Language: Chinese; English
• Published: 05.04.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention belongs to the technical field of medical detection, and discloses a rhesus monkey intestinal tract organ model construction and identification method, which comprises: S1, separating and obtaining monkey intestinal mucosa crypts; s2, culture and passage of monkey intestinal organs; s3, performing immunofluorescent staining identification on the intestinal organs. The form of the constructed rhesus monkey intestinal tract organoid is consistent with that of mouse intestinal tract organoid and human intestinal tract organoid reported in existing literatures, an immunofluorescence result shows that staining of intestinal tract stem cells, intestinal tract epithelial cells AldolaseB + AldolaseC, intestinal tract endocrine cells, panning cells and goblet cells in normal intestinal tract epithelial cell types is positive, and staining of the intestinal tract stem cells, the intestinal tract epithelial cells AldolaseB + AldolaseC, the intestinal tract endocrine cells, the panning cells and the goblet