Amino transferase mutant and method for preparing chiral amine compound

• Main Authors: ZHENG BO, ZHANG YA'NAN, MENG XIAO, XU JIA, WEN XIANGHUI, DAI DANMEI, ZHU JINGYANG, LI HE
• Format: Patent
• Language: Chinese; English
• Published: 02.04.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses an aminotransferase mutant and a method for preparing a chiral amine compound. The invention provides an aminotransferase mutant. The aminotransferase mutant is obtained by performing one or more mutations on a site of an amino acid sequence as shown in SEQ ID NO: 1. The invention also provides a method for preparing the chiral amine compound from the aminotransferase or the aminotransferase mutant, which comprises the following step of: in the presence of the aminotransferase or the aminotransferase mutant, carrying out transamination reaction on a compound shown as a formula II and an amino donor in a solvent to obtain a compound shown as a formula I. The invention also provides a method for preparing the chiral amine compound from the aminotransferase or the aminotransferase mutant. The method for preparing the chiral amine compound by using the aminotransferase or the aminotransferase mutant has one or more of the following advantages: mild conditions, high selectivity, low cost, h