Virus polypeptide-protein subunit combined vaccine based on DNA nanotechnology as well as preparation method and application of virus polypeptide-protein subunit combined vaccine

• Main Authors: SUN LELE, ZHANG YALING, QU YANFEI, YAN LINGLING, ZHANG YUNLONG
• Format: Patent
• Language: Chinese; English
• Published: 02.04.2024
• Subjects: HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS

The invention provides a virus polypeptide-protein subunit combined vaccine based on a DNA (Deoxyribose Nucleic Acid) nanotechnology as well as a preparation method and application of the virus polypeptide-protein subunit combined vaccine, and belongs to the technical field of biological products. According to the virus polypeptide-protein subunit combined vaccine based on the DNA nanotechnology, tetrahedral framework nucleic acid formed by assembling DNA chains is used as a carrier, one edge of the tetrahedral framework nucleic acid is coupled with a protein antigen causing activation of virus specific T cells, and four vertexes of the tetrahedral framework nucleic acid are coupled with virus B cell epitope peptides. In the virus subunit-peptide vaccine of all framework nucleic acids, the SARS-Cvo-2N protein is coupled to a tetrahedral framework, so that CD4 + T cell activation can be caused in various animal models, higher antibody titer is induced, the proportion of memory B cells and T cells is increased,