Ecological cavernous body nitrogen removal efficiency diagnosis method based on metagenome technology

• Main Authors: YU KE, HAN RUITIAN, XIA CHENXI, HU JINCHENG, FAN WENBO, ZHANG YU, QIN HUAPENG
• Format: Patent
• Language: Chinese; English
• Published: 29.03.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

The invention discloses an ecological cavernous body nitrogen removal efficiency diagnosis method based on a metagenome technology. The method comprises the following steps: performing high-throughput sequencing on a microbial 16SrRNA gene in the bioretention system, analyzing the composition and structure of a microbial community in the bioretention system under different plant varieties, soil matrix proportions and pollution loads, and researching the influence of different plant varieties, soil matrix proportions and pollution loads on the abundance of the microbial community and nitrogen functional genes; the relation between the microbial characteristics of the bioretention system and the nitrogen removal rate is established through correlation analysis, regression analysis and other methods, so that the nitrogen removal efficiency of the bioretention system is predicted by detecting the microbial characteristics. By exploring the influence of typical denitrification factors on the nitrogen removal effic