Detection method for vitamin K1 and/or vitamin K2 in health care product and application

• Main Authors: ZHANG MEIJIN, XU ZHENGHUA, ZHONG YU, ZHAO YONG, FANG HAIQIN, XU JUAN, YI YINHUI
• Format: Patent
• Language: Chinese; English
• Published: 29.03.2024
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to a method for detecting vitamin K1 and/or vitamin K2 in health care products and application, and relates to the technical field of food detection. Comprising the following steps: extraction: adding part of an extracting agent into a to-be-detected sample, mixing, extracting and centrifuging to obtain a supernatant and a residual solution, adding the residual extracting agent into the residual solution, mixing, extracting and centrifuging to obtain an extracting solution, merging the extracting solution and the supernatant, adding a solvent to a constant volume, and mixing to obtain a to-be-detected solution; the extracting agent comprises at least one of ethanol, normal hexane and dichloromethane; and detection: performing high performance liquid chromatography detection on the to-be-detected liquid. The detection method not only can be suitable for various dosage forms of health-care products such as tablets, granules, capsules and soft sweets, but also can be used for respectively o