RT-PCR (Reverse Transcription-Polymerase Chain Reaction) primer for identifying porcine rotavirus G4, G5 and G9 type strains and detection method thereof

• Main Authors: LUO XIUXIN, ZHOU MINGGUANG, CAO YI, LI JIA'AI, LI SHUAI, ZHANG HUAWEI, XU GAOYUAN, LIU HUI, CHEN YINGJIN
• Format: Patent
• Language: Chinese; English
• Published: 29.03.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the field of biotechnology detection, and provides RT-PCR detection primers and a method for G4, G5 and G9 strains in porcine rotavirus trivalent attenuated vaccines (G4, G5 and G9), the genotypes of the G4, G5 and G9 strains in the porcine rotavirus trivalent attenuated vaccines can be rapidly identified, and the genotypes of the G4, G5 and G9 strains in the porcine rotavirus trivalent attenuated vaccines can be rapidly identified by designing the targeted primers and a test reaction system, so that the genotypes of the G4, G5 and G9 strains in the porcine rotavirus trivalent attenuated vaccines can be rapidly identified. The virus type can be identified by analyzing a result strip, the detection period can be greatly shortened, the detection is accurate and rapid, and the method has the advantages of simplicity in operation, high sensitivity, rapid detection and the like. 本发明属于生物技术检测领域，通过提供一种针对猪轮状病毒三价弱毒疫苗(G4、G5和G9)中G4、G5和G9型毒株的RT-PCR检测引物及方法，能快速鉴别猪轮状病毒三价弱毒疫苗中G4、G5和G9毒株基因型，该方法通过设计针对性引