Crystal gel adsorption chromatography separation method of lactoferrin

• Main Authors: ZHANG SONGHONG, YIN FEIFAN, ZHANG WENJING, YUN JUNXIAN, LOU XIAOLING, SHEN SHAOCHUAN
• Format: Patent
• Language: Chinese; English
• Published: 29.03.2024
• Subjects: CHEMISTRY; COMPOSITIONS BASED THEREON; MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONSONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS; METALLURGY; ORGANIC CHEMISTRY; ORGANIC MACROMOLECULAR COMPOUNDS; PEPTIDES; THEIR PREPARATION OR CHEMICAL WORKING-UP

The invention discloses a cryogel adsorption chromatography separation method of lactoferrin, which comprises the following steps: preparing a cryogel skeleton by taking chitosan and poly (butyl methacrylate) nanogel as monomers through polymerization, grafting a functional monomer with a sulfonic group to the cryogel skeleton to obtain chitosan-poly (butyl methacrylate) nanocrystal gel, and separating the lactoferrin by taking the chitosan-poly (butyl methacrylate) nanocrystal gel as a separation medium to obtain the lactoferrin-poly (butyl methacrylate) nanocrystal gel. Lactoferrin is separated from whey by chromatography through cryogel adsorption. The lactoferrin is separated through the method, the purity of the obtained lactoferrin can reach 94.6%, the yield can reach 91.3%, and preparation of a high-quality lactoferrin product is facilitated. The chitosan polybutyl methacrylate nanocrystal gel is large in porosity and multiple in adsorption sites, can efficiently adsorb, chromatography and separate lac