Application of low-temperature sensitive type rice zebra leaf gene OsLSZ1 in regulation and control of rice leaf color

• Main Authors: PAN GANG, ZHANG JIE, ZHAO JIAYI, XIANG LONGYI, CHEN JIAMENG, JIN ZEYAN, CHENG FANGMIN
• Format: Patent
• Language: Chinese; English
• Published: 26.03.2024
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention belongs to the field of rice breeding, and particularly relates to application of a low-temperature sensitive type rice zebra leaf gene OsLSZ1 in regulation and control of rice leaf color, and the genome sequence of the low-temperature sensitive type rice zebra leaf gene OsLSZ1 is shown as SEQ ID NO.1. According to the invention, the OsLSZ1 gene is cloned from a rice zebra leaf mutant oslsz1 through map-based cloning. A genetic complementary expression vector is constructed and introduced into the mutant oslsz1, and the transgenic offspring shows a normal leaf color, thereby confirming that the zebra leaf symptom in the seedling stage of the mutant oslsz1 is caused by single base substitution of the OsLSZ1 gene, and indicating that the OsLSZ1 gene can control early development of chloroplast of rice leaves. Therefore, rice chloroplast development is regulated and controlled based on the oslsz1 gene product, so that the marker character of zebra leaves in the seedling stage is generated, and the