Recombinant plasmid and recombinant engineering bacterium for expressing white kidney bean Alpha-amylase inhibitory protein and preparation method of recombinant plasmid and recombinant engineering bacterium

The invention discloses a recombinant plasmid for expressing white kidney bean Alpha-amylase inhibitory protein, a recombinant engineering bacterium and a preparation method of the recombinant plasmid. The white kidney bean Alpha-amylase inhibitory protein is obtained by a prokaryote fermentation me...

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Main Authors LIN JIACHUN, LIAO JIALONG, LONG JUNRU, HUANG QIANG, GAO CHUHAN, YUAN YUE, WANG WENZHAO
Format Patent
LanguageChinese
English
Published 26.03.2024
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses a recombinant plasmid for expressing white kidney bean Alpha-amylase inhibitory protein, a recombinant engineering bacterium and a preparation method of the recombinant plasmid. The white kidney bean Alpha-amylase inhibitory protein is obtained by a prokaryote fermentation method. The method comprises the following steps: extracting white kidney bean Alpha-amylase inhibitor protein genes, and performing codon optimization on the white kidney bean Alpha-amylase inhibitor protein genes respectively to construct a first white kidney bean Alpha-amylase inhibitor protein gene (PASI01) and a second white kidney bean Alpha-amylase inhibitor protein gene (PASI02) respectively; a first recombinant plasmid (pET28a-PASI01), a first recombinant engineering bacterium (DE3-pET28a-PASI01), a second recombinant plasmid (pHY-P43-PASI02) and a second recombinant engineering bacterium (WB800N-pHY-P43-PASI02) are respectively constructed, enzyme activity detection and inhibition rate test are carried out
Bibliography:Application Number: CN202410162793