Penicillium digitatum transcription factor PDIDSM85260 and application thereof

• Main Authors: ZHANG LULU, LIU KUNLUN, GAO ZIQING, CHEN YAN, ZHANG JIAYUE, LI HUA, LI ZEHAO, HUANG JIALIN, YANG JINCHU, ZHAO BEIBEI
• Format: Patent
• Language: Chinese; English
• Published: 26.03.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to a penicillium digitatum transcription factor PDIDSM85260 and an application of the penicillium digitatum transcription factor PDIDSM85260. The nucleotide sequence of the Penicillium digitatum transcription factor PDIDSM85260 is as shown in SEQ ID No. 1, and the amino acid sequence of the protein coded by the Penicillium digitatum transcription factor PDIDSM85260 gene is as shown in SEQ ID No. 2. The yield of the alpha-terpilenol obtained by biotransformation of the PDIDSM85260 gene overexpression strain is 1870-2798 mg L <-1 >, which is reported at present that the yield of the alpha-terpilenol prepared by directly using microorganisms for transformation is higher, and the problem that the yield of the alpha-terpilenol produced by biotransformation of limonene is low is solved. 本发明涉及一种指状青霉转录因子PDIDSM_85260及其应用。该指状青霉转录因子PDIDSM_85260的核苷酸序列如SEQ ID No.1所示，所述指状青霉转录因子PDIDSM_85260基因编码的蛋白的氨基酸序列如SEQ ID No.2所示。本发明利用PDIDSM_85260基因过表达菌株生物转化得到的α-松油醇的产量为1870～2798mg L-1，是目前所报道直接利用微生物进行转化制得α-松油醇中产量较高的