Enzyme and cofactor co-immobilized millimeter sphere catalyst as well as preparation and application thereof

• Main Authors: YANG HENGQUAN, YOO HEON, HAO RUIPENG, FAN MIN
• Format: Patent
• Language: Chinese; English
• Published: 26.03.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the field of preparation of catalytic materials, and particularly relates to an enzyme and cofactor co-immobilized millimeter sphere catalyst as well as preparation and application thereof. The particle size of each millimeter sphere is 1.0-5.0 mm, the enzyme content is 7.9-72.1 mg/g, the cofactor content is 9.9-39.5 mg/g, and the mechanical strength is 3-12 N. The preparation method comprises the following steps: adding solid nanoparticles, biological enzyme and cofactors into an aqueous solution of amine, uniformly stirring to prepare a mixed solution, continuously dripping the mixed solution on a layer of hydrophobic nano silicon oxide powder flatly laid on a flat surface, and drying to obtain the hydrophobic nano silicon oxide/biological enzyme composite material. Rolling to form a droplet millimeter ball with a silicon oxide shell; collecting liquid drop millimeter balls, and slowly drying to finally obtain enzyme and cofactor co-immobilized millimeter balls. The enzyme and cofac