Imine reductase mutant, imine reductase and glucose dehydrogenase co-expression enzyme and application of imine reductase and glucose dehydrogenase co-expression enzyme

• Main Author: CHEN ZECONG
• Format: Patent
• Language: Chinese; English
• Published: 22.03.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses an imine reductase mutant and a preparation method of the imine reductase mutant for preparing (S)-nicotine. The first purpose of the invention is to provide an imine reductase mutant to solve the problems that nicotine with S configuration cannot be obtained by using an enzyme in the prior art and the enzyme activity is low in the prior art, and the mutant can catalytically reduce a substrate, i.e., Melisine or 4-(methylamino)-1-(pyridine-3-yl) butanone into (S)-nicotine. The second purpose of the invention is to provide the co-expression enzyme of the imine reductase and the glucose dehydrogenase, in particular to the co-expression enzyme of the imine reductase mutant enzyme and the glucose dehydrogenase, the co-expression enzyme is applied to preparation of (S)-nicotine, and the cost of preparing the (S)-nicotine through enzyme catalysis is reduced. 本发明公开了一种亚胺还原酶突变体，以及这种亚胺还原酶突变体用于制备(S)-尼古丁的制备方法。发明第一个目的是提供了一种亚胺还原酶突变体，解决现有技术使用酶得不到S构型的尼古丁，以及现有技术中酶活性低的问题，这种突变体可以将底物麦思明或4-(甲胺基)-1-(吡啶-3-基)