Method for detecting saponin content in chenopodium quinoa willd grains by hydrochloric acid solution shaking foaming method

• Main Authors: ZHANG XIAOYING, ZHANG LIHONG, QU QIANWEN, YANG YANDUO, ZHOU XUEYONG, ZHANG ZIKUAN
• Format: Patent
• Language: Chinese; English
• Published: 22.03.2024
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides a method for detecting saponin content in chenopodium quinoa willd grains by using a hydrochloric acid solution shaking foaming method. Comprising the following steps: preparing a hydrochloric acid solution with the concentration of 0.001-0.006 mol/L; weighing the chenopodium quinoa willd grains; determining the saponin content of the quinoa grains: putting the weighed quinoa grains into a test tube, adding a hydrochloric acid solution, covering a plug, soaking at room temperature, holding the test tube with a hand, violently shaking up and down for 40 times, and standing for 1 minute; and calculating the content of the quinoa saponin by utilizing a conversion formula between the height of the upper-layer foam, the height of the lower-layer liquid, the total height of the upper-layer foam and the lower-layer liquid and the concentration of the quinoa saponin. According to the method, formulas for calculating the saponin content by the upper-layer foam height, the lower-layer liquid leve