IDH multi-mutation subtype detection method and kit

• Main Authors: LIU SISI, TANG YUANBIN, SHAO YANG, CHENG XI, MENG SUFANG, ZHANG ANDU, NA CHENGLONG
• Format: Patent
• Language: Chinese; English
• Published: 22.03.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a method for simultaneously detecting multi-subtype gene locus mutation in isocitrate dehydrogenase 1 (IDH1) or isocitrate dehydrogenase 2 (IDH2) and a reagent thereof, the method is based on an ARMS fluorescent quantitative PCR (polymerase chain reaction) method, and the mutation of multiple subtype gene loci in isocitrate dehydrogenase 1 (IDH1) or isocitrate dehydrogenase 2 (IDH2) is detected in the same reaction system. And detecting a to-be-detected sample by using ARMS primers of a mutant template aiming at the IDH1 or IDH2 gene mutation site and an upstream or downstream primer shared by the ARMS primers. The method provided by the invention is simple and convenient to operate, and can detect that a sample contains an IDH mutation site through multiple detection in a short time. 本发明提供一种同时检测异柠檬酸脱氢酶1(IDH1)或者异柠檬酸脱氢酶2(IDH2)中多亚型基因位点突变的方法及其试剂，所述方法基于ARMS荧光定量PCR方法，在相同的反应体系中，分别使用针对所述IDH1或IDH2基因突变位点的突变型模板的ARMS引物及它们共用的上游或下游引物对待测样本进行检测。本发明的方法操作简便，可在短时间内通过多重检测，检出样本中是含有IDH突变位点。