Sphingolipid molecular bioreceptor as well as preparation method and application thereof

• Main Authors: YAO NAN, LI YONGKANG, DAI GUANGYI, ZHANG YUMENG
• Format: Patent
• Language: Chinese; English
• Published: 22.03.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DIAGNOSIS; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; IDENTIFICATION; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; SURGERY; TESTING; VINEGAR; WINE

The invention relates to the technical field of biology, and discloses a sphingolipid molecular bioreceptor as well as a preparation method and application thereof. The bioreceptor at least comprises a donor fluorescent protein, a to-be-detected substance binding protein, a receptor fluorescent protein and a membrane positioning signal peptide, wherein the C end of the donor fluorescent protein is connected with the N end of the to-be-detected substance binding protein through a first connecting arm, and the C end of the to-be-detected substance binding protein is connected with the N end of the receptor fluorescent protein through a second connecting arm; the C end of the receptor fluorescent protein is connected with the N end of the membrane positioning signal peptide; and the second connecting arm comprises at least one flexible joint. The bioreceptor provided by the invention can realize multidirectional rotation, and can perform efficient and sensitive dynamic monitoring on specific molecules (such as s