Combined detection reagent and kit for cervical cancer detection

• Main Authors: YANG DONGCHENG, LI MENGZHEN, CHEN JINGCHEN, SONG LAISI, WU HUILIAN, DENG YANGYANG, ZHANG QIAOSHI, LI SHILIANG, CAI XINGSHENG
• Format: Patent
• Language: Chinese; English
• Published: 19.03.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of gene diagnosis, in particular to a combined detection reagent and kit for cervical cancer detection. The method comprises the following steps: designing a detection reagent for detecting methylation of a cervical cancer SOX1 gene, an SEPTIN9 gene, a TAC1 gene and a ZIC1 gene; according to the method, four HPVs (Human Papilloma Virus 16/18/52/58) with relatively high detection specificity and detection sensitivity are screened and confirmed, a detection reagent for detecting HPV16/18/52/58 virus methylation is designed, and any combination of SOX1/SEPTIN9/TAC1 or SOX1/SEPTIN9/ZIC1 is combined with HPV16/18/52/58 virus methylation detection, so that high-specificity and high-sensitivity detection on cervical cancer and precancerous lesions thereof can be realized; and the method is suitable for large-scale population screening and conventional molecular diagnosis. 本发明涉及基因诊断的技术领域，尤其是涉及一种用于宫颈癌检测的组合检测试剂、试剂盒；本公开通过设计检测宫颈癌SOX1基因、SEPTIN9基因、TAC1基因、ZIC1基因甲基化的检测试剂；再筛选确认具有较高