Specific high-throughput sequencing method for single-stranded DNA based on 5 'connection
The invention belongs to the technical field of biology, and particularly relates to a specific high-throughput sequencing method for single-stranded DNA based on 5'connection. The method specifically comprises a method for preparing a single-stranded DNA sequencing library based on 5 '-te...
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Main Authors | , , |
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Format | Patent |
Language | Chinese English |
Published |
15.03.2024
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Subjects | |
Online Access | Get full text |
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Summary: | The invention belongs to the technical field of biology, and particularly relates to a specific high-throughput sequencing method for single-stranded DNA based on 5'connection. The method specifically comprises a method for preparing a single-stranded DNA sequencing library based on 5 '-terminal connection. The method comprises the following steps for treating a sample to be detected: step 1) supplementing the 5'-terminal of dsDNA with DNA polymerase without exonuclease activity; the method comprises the following steps of (1) carrying out 3'terminal tailing, (2) carrying out 3 'terminal tailing, (3) connecting a 5'-stem-loop joint subjected to renaturation treatment, (4) carrying out amplification on a connection product, and (5) carrying out amplification on the connection product to form a single-stranded DNA sequencing library, wherein the structure of the 5 '-stem-loop joint is as follows: a protruding terminal, a random base region, a first stem region, a loop region and a second stem region, and the fi |
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Bibliography: | Application Number: CN202410166121 |