ITGB5 conditional gene knockout mouse model, construction method and application thereof

• Main Authors: TANG HUI, FAN QINGHUA, LI XIAOMIN, ZENG YONGQING, LI MINGHUI, MA XIAOYUN, ZHANG QIN, WANG WENWEN, FENG YULIAN
• Format: Patent
• Language: Chinese; English
• Published: 15.03.2024
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW BREEDS OF ANIMALS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses an ITGB5 conditional gene knockout mouse model as well as a construction method and application thereof, and belongs to the technical field of gene engineering. According to the invention, Cas9 mRNA, gRNA and a homologous recombinant vector are microinjected into a fertilized egg of a C57BL/6J mouse, and the fertilized egg is transplanted to a pseudopregnant mouse to obtain an F0-generation mouse; hybridizing the F0 generation mouse containing the targeting sequence with a wild type mouse to obtain a heterozygous mouse containing the targeting sequence; and hybridizing the obtained heterozygous mouse containing the targeting sequence with a Vil-Cre positive mouse to obtain the animal model with the intestinal ITGB5 gene knocked out. According to the present invention, the conditional Knockout (CKO) mouse is obtained, and the convenient, reliable and economic animal model is provided for the research on the influence of the ITGB5 gene on the resistance of the intestinal tract on the ETE