Moringa oleifera leaf polysaccharide extraction process

• Main Authors: ZHOU WEIHUA, LIU SHUCHENG, WANG GUOHAO, CAO CHUNHUA, LEI YANG, JIANG QIONG, ZHONG HUAYU
• Format: Patent
• Language: Chinese; English
• Published: 15.03.2024
• Subjects: CHEMISTRY; COMPOSITIONS BASED THEREON; DERIVATIVES THEREOF; METALLURGY; ORGANIC MACROMOLECULAR COMPOUNDS; POLYSACCHARIDES; THEIR PREPARATION OR CHEMICAL WORKING-UP

The invention discloses a moringa oleifera leaf polysaccharide extraction process, and relates to the technical field of moringa oleifera leaf polysaccharide extraction, and the technical key point is that the moringa oleifera leaf polysaccharide extraction process comprises the following steps: S1, weighing moringa oleifera leaf powder, carrying out reflux degreasing with petroleum ether, then adding distilled water with a certain material-liquid ratio, adding pectinase, cellulase and papain according to a ratio, and adjusting the pH value; s2, then carrying out ultrasonic treatment, carrying out rotary evaporation on the filtrate after ultrasonic treatment is finished, and carrying out protein removal on the concentrated solution after rotary evaporation by adopting a Sevage method; s3, after protein is removed, supernate is taken, the volume is weighed, ethyl alcohol with the volume 4 times that of the supernate is added, then polysaccharide is precipitated overnight at the temperature of 4 DEG C and centr