Method for measuring protein C and activated protein C

• Main Authors: CARBONELL MONLEON NEVES, PALLARDO CALATAYUD FEDERICO VICENTE, MENA MORA SALVADOR, GARCIA GIMENEZ JOSE LUIS
• Format: Patent
• Language: Chinese; English
• Published: 08.03.2024
• Subjects: HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEDICAL OR VETERINARY SCIENCE; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; TESTING

The present invention relates to a method of measuring the level of Protein C (PROC), preferably Activated Protein C (APC), in a subject in need thereof, comprising: a step of enzymatic digestion of one or more biological samples isolated from the subject; and a step of measuring the level of the peptide of SEQ ID NO: 1 (LGEYDLR and/or of the peptide of SEQ ID NO: 2 (TFVLNFIK), wherein the level of SEQ ID NO: 1 and/or of SEQ ID NO: 2 corresponds to the level of PROC, preferably APC, present in said one or more biological samples. Methods of classifying and prognosing a therapeutic response in a subject are also included. 本发明涉及一种测量有需要的对象中蛋白C(PROC)、优选活化蛋白C(APC)水平的方法，该方法包括：对从对象分离的一个或多个生物样本进行酶促消化的步骤；和测量SEQ ID NO：1(LGEYDLR)的肽和/或SEQ ID NO：2(TFVLNFIK)的肽的水平的步骤，其中SEQ ID NO：1和/或SEQ ID NO：2的水平对应于所述一个或多个生物样本中存在的PROC、优选APC的水平。还包括对对象进行分类和预后治疗反应的方法。