Application of PsLHCB5 gene in regulation and control of peony green flowers and fruiting

• Main Authors: SHU QINGYAN, LU GAOJIE, LI YANG, HAO QING, PENG LIPING, LI TONGTONG
• Format: Patent
• Language: Chinese; English
• Published: 08.03.2024
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; ORGANIC CHEMISTRY; PEPTIDES; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses application of a PsLHCB5 gene in regulation and control of peony green flowers and fruiting, and belongs to the field of gene engineering. According to the invention, a PsLHCB5 gene coding sequence is obtained, and biological functions of candidate genes are verified through tobacco overexpression transformation and virus-induced gene silencing by an agrobacterium-mediated method. Compared with a wild type plant, the PsLHCB5 transgenic line overexpressed has phenotypic characters such as increased plant height, increased crown breadth, stronger growth vigor, increased number of inflorescence branches, increased fruiting amount, deepened leaf color and the like, but the initial color of petals is lighter than that of the wild type plant, and the total chlorophyll content of the petals is reduced after PsLHCB5 is silenced in the peony. The result shows that the PsLHCB5 can positively regulate and control the synthesis of the peony chlorophyll, and further proves the effect of the PsLHCB5