Diaphorina citri salivary gland gene DcS21 as well as encoding protein and application thereof

• Main Authors: ZHOU GUCHUN, KUANG YINHUI, CHEN ZHIQIN, YU XIUDAO, SHANGGUAN CHAOZHI
• Format: Patent
• Language: Chinese; English
• Published: 08.03.2024
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses a diaphorina citri salivary gland gene DcS21, an encoding protein and application thereof, and aims to solve the technical problem that diaphorina citri is difficult to harmlessly control. The diaphorina citri salivary gland gene DcS21 is obtained through screening, a salivary gland transcriptome obtained from diaphorina citri is obtained, the nucleotide sequence of the diaphorina citri salivary gland transcriptome is as shown in SEQ ID NO.1, and the encoded amino acid sequence is as shown in SEQ ID NO.2. The diaphorina citri salivary gland gene DcS21 is a salivary gland transcriptome. After the silent DcS21 gene is treated by dsRNA, the viability of diaphorina citri and the pathogenicity of the diaphorina citri to a host citrus are remarkably reduced compared with those of a control GFP dsRNA treatment group, which indicates that the gene plays an important role in feeding and infection pathogenicity processes of diaphorina citri and can be used as a target gene of plant diaphorina ci